Coding

Part:BBa_K2424001:Design

Designed by: Eirik Bager Sundmark   Group: iGEM17_UiOslo_Norway   (2017-10-22)


S. Pombe codon optimzed superfolder GFP (sfGFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The biggest design consideration with this part was the optimization, and measuring how effective that optimization is compared to other sfGFPs expressed in S. Pombe would be beneficial.


Source

The sequence was created by taking an sfGFP-sequence reportedly used for successful transformation of E. Coli TOP10-cells, and codon-optimized through use of IDT's codon optimization tool: https://eu.idtdna.com/CodonOpt

References